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recombinant human macrophage colony  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human macrophage colony
    Recombinant Human Macrophage Colony, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+macrophage+colony/Recombinant+Human+M-CSF+Protein/pmc13068806-164-39-45
    Average 96 stars, based on 604 article reviews
    recombinant human macrophage colony - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: SHIV.D Infection Alters Production and Protein Composition of Myeloid-Derived Extracellular Vesicles
    Article Snippet: RM peripheral blood mononuclear cells (PBMCs) were isolated from EDTA whole blood using a Ficoll (Cytiva, Marlborough, MA, USA) gradient and plated in RPMI medium (ThermoFisher Scientific, Waltham, MA, USA) + 10% exosome-depleted FBS (ThermoFisher Scientific, Waltham, MA, USA) + gentamicin (Corning, Corning, NY, USA) across 6-well plates at a density of 1 million cells/mL.

    In Vitro:

    Article Title: Interleukin-34 orchestrates bone formation through its binding to Bone Morphogenic Proteins
    Article Snippet: .. In vitro experiments Reagents Recombinant human Macrophage-Colony Stimulating Factor (M-CSF), human interleukin-34 (IL-34), human M-CSF receptor (M-CSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP-2), human bone morphogenetic protein 4 (BMP-4), human bone morphogenetic protein 7 (BMP-7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK). .. Anti-human IL34 (BT-34) mouse IgG1 monoclonal antibody was produced by Diaclone (Besançon, France) under patent (Heymann D, Ségaliny A, Brion R. University of Nantes /Nantes Hospital/INSERM, “Anti-IL-34 antibodies”.

    Recombinant:

    Article Title: Interleukin-34 orchestrates bone formation through its binding to Bone Morphogenic Proteins
    Article Snippet: .. In vitro experiments Reagents Recombinant human Macrophage-Colony Stimulating Factor (M-CSF), human interleukin-34 (IL-34), human M-CSF receptor (M-CSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP-2), human bone morphogenetic protein 4 (BMP-4), human bone morphogenetic protein 7 (BMP-7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK). .. Anti-human IL34 (BT-34) mouse IgG1 monoclonal antibody was produced by Diaclone (Besançon, France) under patent (Heymann D, Ségaliny A, Brion R. University of Nantes /Nantes Hospital/INSERM, “Anti-IL-34 antibodies”.

    Article Title: The unique lipopolysaccharide composition of Salmonella Typhimurium ST313 dampens pyroptosis and inflammasome activation and suppresses host cell death
    Article Snippet: This was followed by adhesion on plastic at 37°C for 2h in adhesion medium (RPMI 1640 (Life Technologies) supplemented with 100 μg/ml streptomycin/penicillin and 2mM L- glutamine (Invitrogen/Gibco)). .. Then, the adhered cells were washed once with warm adhesion medium and differentiated in complete macrophage medium (RPMI 1640 supplemented with 10% FCS (Eurobio), 100 μg/ml streptomycin/penicillin, 2 mM L-glutamine) and 10 ng/mL recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems) [ , ]. .. Bone marrow-derived macrophages were differentiated in DMEM (Gibco) supplemented with 20% MCSF (3T3 supernatant), 10% heat-inactivated FCS (Bioconcept), 10 mM HEPES (Bioconcept), 100 μg/ml penicillin/streptomycin (Bioconcept, 4-01H00-H) and non-essential amino acids (Gibco, 11140-035 at 1/100), and stimulated on day 7 – 9 of differentiation as in [ ].

    Article Title: SHIV.D Infection Alters Production and Protein Composition of Myeloid-Derived Extracellular Vesicles.
    Article Snippet: RM peripheral blood mononuclear cells (PBMCs) were isolated from EDTA whole blood using a Ficoll (Cytiva, Marlborough, MA, USA) gradient and plated in RPMI medium (ThermoFisher Scientific, Waltham, MA, USA) + 10% exosome-depleted FBS (ThermoFisher Scientific, Waltham, MA, USA) + gentamicin (Corning, Corning, NY, USA) across 6-well plates at a density of 1 million cells/mL. .. After 24 h in culture, differentiation into monocyte-derived macrophages (MDM) was stimulated with 50 ng/mL recombinant human macrophage colony-stimulating factor (M-CSF (R&D Systems, Minneapolis, MN, USA)). .. RM peripheral blood mononuclear cells (PBMCs) were isolated from EDTA whole blood using a Ficoll (Cytiva, Marlborough, MA, USA) gradient and plated in RPMI medium (ThermoFisher Scientific, Waltham, MA, USA) + 10% exosome-depleted FBS (ThermoFisher Scientific, Waltham, MA, USA) + gentamicin (Corning, Corning, NY, USA) across 6-well plates at a density of 1 million cells/mL.

    Article Title: Interleukin-34 orchestrates bone formation through its binding to bone morphogenic proteins
    Article Snippet: .. Recombinant human Macrophage-Colony Stimulating Factor (MCSF), human interleukin-34 (IL34), human M-CSF receptor (MCSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP2), human bone morphogenetic protein 4 (BMP4), human bone morphogenetic protein 7 (BMP7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK). .. Anti-human IL34 (BT-34) mouse IgG1 monoclonal antibody was produced by Diaclone (Besançon, France) under patent (Heymann D, Ségaliny A, Brion R. University of Nantes /Nantes Hospital/INSERM, “Anti-IL-34 antibodies”.

    Article Title: Immunomodulatory, anti-synoviocyte, and anti-osteoclastic abilities of embryonic stem cell-derived mesenchymal stem cells in rheumatoid arthritis.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: HIV-1 and Chlamydia trachomatis restrict their respective growth but promote their survival in co-infected human macrophages
    Article Snippet: Human monocytes were isolated from peripheral blood of healthy donors (Etablissement Français du Sang Ile-de-France, Site Trinité, Inserm agreement #18/EFS/030 ensuring that all donors gave a written informed consent and providing anonymized samples) by density gradient sedimentation on Ficoll (GE Healthcare), followed by adhesion to plastic at 37 °C for 2 h in the presence of adhesion medium (RPMI 1640 (Life Technologies) supplemented with 100 μg/ml streptomycin/penicillin and 2 mM L-glutamine (Invitrogen/Gibco). .. Then, the adhered cells were washed once with warm adhesion medium and differentiated in macrophage medium (RPMI 1640 supplemented with 10% FCS (Eurobio), 100 μg/ml streptomycin/penicillin, 2 mM L-glutamine) and 10 ng/mL recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems) for 6-7 days. .. i) C. trachomatis Lymphogranuloma venereum, Type II was from ATCC (L2/434/Bu VR-902B) and the fluorescent strains p2TK2-SW2 IncDProm-RSGFP-IncDTerm (GFP-Ct) and p2TK2-SW2 IncDProm-mCherry-IncDTerm (mCherry-Ct) prepared by Hervé Agaisse and Isabelle Derré (Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America) were kindly given by Agathe Subtil (Institut Pasteur, Paris).

    Article Title: Biomimetic bone calcium phosphate-based scaffolds fabricated via ceramic vat photopolymerization: Effect of porosity, sintering temperature, mineralogical phases and trace elements on the osteogenic potential
    Article Snippet: .. Monocytes were plated at a density of 1.5 × 10 5 cells/cm 2 in α-MEM containing 10% fetal bovine serum (FBS, Sigma-Aldrich), and 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone S.p.A., Pero, Italy) further supplemented with 25 ng/mL recombinant human macrophage colony-stimulating factor (M-CSF, R&D systems, Minneapolis, MN, USA), and 25 ng/mL recombinant human receptor activator of nuclear factor κ-B ligand (RANK-L, Gibco, Thermo Fisher Scientific). ..

    Cell Culture:

    Article Title: Immunomodulatory, anti-synoviocyte, and anti-osteoclastic abilities of embryonic stem cell-derived mesenchymal stem cells in rheumatoid arthritis.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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